Differential Modulation of Porcine Theca, Granulosa, and Luteal Cell Steroidogenesis in Vitro by Tumor Necrosis Factor1
نویسندگان
چکیده
منابع مشابه
Luteal macrophage conditioned medium affects steroidogenesis in porcine granulosa cells.
The purpose of the study was to examine the effect of luteal macrophage conditioned medium (LMCM) on progesterone and estradiol production by cultured granulosa cells. Porcine granulosa cells were cultured for 48 h with or without LMCM in the absence or presence of 100 ng/ml LH, FSH or prolactin. Progesterone and estradiol concentrations were measured by radioimmunoassay. Granulosa cells were a...
متن کاملIn Vitro Effect of Triiodothyronine on the Cyclic AMP, Progesterone and Testosterone Level in Porcine Theca, Granulosa and Luteal Cells.
OBJECTIVE: To investigate the influence of thyroid hormone on steroid production and cAMP accumulation in porcine theca (Tc) and granulosa cells (Gc) isolated from preovulatory follicles as well as in luteal cells isolated during the mid-developing luteal phase. METHODS: Granulosa and theca cells separated from pig ovarian follicles and pieces of corpora lutea were cultured in the incubation me...
متن کاملParacrine Regulation of Steroidogenesis in Theca Cells by Granulosa Cells Derived from Mouse Preantral Follicles
Interaction partners of follicular cells play a significant role in steroidogenesis, follicular formation, and development. Androgen secreted by theca cells (TCs) can initiate follicle development and ovulation and provide precursor materials for estrogen synthesis. Therefore, studies on ovarian microenvironment will not only lead to better understanding of the steroidogenesis but also have cli...
متن کاملParacrine effects of oocyte secreted factors and stem cell factor on porcine granulosa and theca cells in vitro
Oocyte control of granulosa and theca cell function may be mediated by several growth factors via a local feedback loop(s) between these cell types. This study examined both the role of oocyte-secreted factors on granulosa and thecal cells, cultured independently and in co-culture, and the effect of stem cell factor (SCF); a granulosa cell derived peptide that appears to have multiple roles in ...
متن کاملThe Anti-Epileptic Drug Valproic Acid (VPA) Inhibits Steroidogenesis in Bovine Theca and Granulosa Cells In Vitro
Valproic acid (VPA) is used widely to treat epilepsy and bipolar disorder. Women undergoing VPA treatment reportedly have an increased incidence of polycystic ovarian syndrome (PCOS)-like symptoms including hyperandrogenism and oligo- or amenorrhoea. To investigate potential direct effects of VPA on ovarian steroidogenesis we used primary bovine theca (TC) and granulosa (GC) cells maintained un...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biology of Reproduction
سال: 1993
ISSN: 0006-3363,1529-7268
DOI: 10.1095/biolreprod48.4.936